Have you used a fluorometric NADPH assay kit with conifer needles?

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How to quench/denature FAM, TdTomato in FFPE tissue sections?

Hi Researchgate folks, Our lab has mouse FFPE tissue which has cells that express a TdTomato reporter as well has a FAM labeled drug as we routinely use to test the delivery of the drug to the...

15 April 2024 6,632 0 View

Passage Max for Human Microvascular Endothelial Cells?

Hello, I am new to working with human microvascular endothelial cells (bought from Sigma), and there is varying information on how many passages you can take these cells to and still get a...

26 September 2023 375 2 View

How can I coat APTES on poly(HEMA)?

I want to coat APTES on pHEMA thin film. I have prepared a Silanization solution (5% APTES in 95% EtOH) and the pH was adjusted to 5 by acetic acid. But it is not work. Can anyone help me provide...

24 May 2023 2,419 2 View

Someone may indicate articles and research programs on Semantic Cluster Analysis, Evolutionary Algorithms and Real-Valued data?

Decolonial Computing is an innovative concept to distinguish the benefits of artificial intelligence in providing economic diversity and inclusion. For this to happen, the world of data analytics...

16 April 2023 7,260 2 View

What is the corresponding reionlatship between forward bias current and solar irradiance ?

Working conditions were simulated by forward biasing the 1 mm2 solar cells at the same current level (250 mA, i.e. 25 A/cm2) they would handle at the operating concentration (i.e. 1000 suns)’

28 February 2023 736 1 View

Which direction does receptors on Golgi apparatus face?

Which way does the receptors on Golgi apparatus face? (facing the cytoplasm or the golgi sacs?) Answering this part above is satisfying enough and I’m all gratitude. I’m asking this because I’m...

17 February 2023 4,277 0 View

How does different neurotransmitters convey different information?

If, let’s say glutamate and dopamine(both excitatory), both causes depolarisation in post synaptic neurons, how do they convey different information?

27 December 2022 8,906 2 View

PerkinElmer CHN 2400 elemental analysis changing carrier gas from Helium to Argon?

Hi Researchers and Scholars We are a chemical research company using CHN 2400 from PerkinElmer for elemental analysis. Due to the Helium global shortage we switch the carrier gas from Helium to...

01 September 2022 1,566 0 View

" ATOM syntax incorrect: "B" is not a valid AutoDock type. Note that AutoDock atom types are case-sensitive " How do I solve this error?

Got this error while performing docking in Pyrx after running vina. ligands and proteins both are optimized can someone help??

25 July 2021 6,473 4 View

Site-specific recombinases for long (>10kb) gene deletions?

I've read that the traditional cre-lox system is limited by the distance between the loxP sites, and is usually only functional up to about 2 kb. I am looking to use a site-specific recombinase...

24 October 2020 1,230 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

Can I separate xylene from paraffin after deparaffinization of microtome samples and reuse the xylene?

...

24 July 2024 1,559 1 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View