EMB is for the isolation, enumeration, and differentiation of members of Enterobacteriaceae. Streptococcus pneumoniae is a fastidious bacteria, and will need blood supplement. Blood agar is best.

the culture of Streptococcus pneumoniae requires an enriched medium such as fresh blood agar at 10%, with antibiotic ANC(nalidixic acid), and you must incubate it in a CO2- enriched atmosphere (24-48h/37°C).

The colonies are alpha hemolytic (partial hemolysis).

We isolated bacteria in the culture medium of EMB that are Streptococcus with gram staining and in biochemical tests as Streptococcus pneumoniae. It was interesting

Yes it's possible, because a culture medium is not ever selective 100%, but as same you need to make a transplanting on blood Agar

EMB is for Gram negative pathogens isolation and S. pneumoniae is gram positive. Use blood agar or Chocolate agar for SPn detection.