Dear colleagues,
Currently I have difficulty to grow S. pneumoniae. Somehow it does not grow well. The OD600nm gets only upto 0.5 - 0.8 after 5 days of innoculation with TSB + 5% CO2
On testing MIC, it does not grow any (OD600nm 0.04) in CAMHB (standardized condition of CLSI)
I would like to ask your protocols of growing S. pneumoniae. Which exact condition you have used to have fully growth during conventional growth phase of bacteria (I mean 16 - 18 hours)
Any suggestions and advices are welcoming
Thanks a lot ,
Reference for my method
Chapter Optimal Conditions for Streptococcus pneumoniae Culture: In ...
Hello,
This article may help you better, is one of the cited documents of your reference article. It also explains the factors that may inhibit these bacteria to growth. The incubation time that you are using may be too long, glucose concentration may need to be reduced, and considerations about microaerophilia, or anaerobiosis may be needed to apply.
Article Optimization of medium and cultivation conditions for capsul...
Hey,
Using Brain heart infusion(BHI) broth or Standard Todd Hewitt broth can be used for culturing S.pneumoniae according to the appended article. You can try using the same for MIC. CLSI does stipulate CAMHB for broth dilution assays, though they talk about supplementing with certain conc. of Lysed horse blood(LHB). Researchers also do modify the protocol to account for growth limitations while using CAMHB. Hope this is helpful. Good luck!
Article Optimization of culture conditions to obtain maximal growth ...
To all answering colleagues,
Thank you for your ideas / experience sharing
It is very helpful for your suggestions. I am preparing those components as you have mentioned. Hopefully I will be successful in innoculating this bacteria
Again, thank you so much !