Hello All,
I am working on finding out binding kinetics of nanoMIPs against and inactivated virus using SPR. I have observed that with decreasing the flow rate of the sample (4 ul/min->2 ul/min->0 ul/min), the rate of association increases.. which is contrary to what literature (and logic) says if my experiment is suffering from Mass transport limitation. It would be really nice if you can shed a light on this?
I am looking forward to your responses.
Thanks
Best regards :)