So, I will be culturing H1 over geltrex, which will be subjected to electroporation, and subsequently FACS-single cell sorting.
I am currently deciding which of these two medium shall I work with..., any suggestions will be very much appreciated! Thanks!!!!!!
if you go with mTeSR1, get mTeSR1+ with temperature-stable bFGF. I use mTeSR for many years and work with hESCs for the past ...18 years, highly recommend. Never heard of stemflex. I attached one useful paper, which compared several types of hESC media, i hope it helps, good luck
We've tested both media for our gene editing experiments on iPSCs. Stemflex (in combination with CloneR (Stemcell)) worked best in our hands. Furthermore, we noticed that iPSCs cultured in Stemflex recover more easily after cryopreservation, or (as mentioned above), after single cell seeding. Good look with your experiments!
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