Dear Tone,
As far as I know, both TrypLe do not have differ significance in their effect on cell viability, if use according to their protocol.
Hi Tone, i can help with any questions as I work with the teams that developed Gibco trypsin and Gibco TrypLE Express/Select.
Firstly, the difference between TrypLE Express and TrypLE Select is that Select is manufactured on animal origin free (AOF) machinery. TrypLE Express is also AOF but not manufactured on AOF machinery. Express and Select are both a single recombinant enzyme.
Our R&D experience from our transfection and primary/stem cell groups is that overall TrypLE is gentler on cells in terms of viability and cell surface antigens. Customers have told us and published that for certain cell types TrypLE is preferred over trypsin.
We have also heard from customers that the fact TrypLE does not need to be freeze/thawed like trypsin makes using it easier :)
Hope this helps and feel free to ask any more questions.
Nicol
@Bjmal I believe i did. Please see "
Firstly, the difference between TrypLE Express and TrypLE Select is that Select is manufactured on animal origin free (AOF) machinery. TrypLE Express is also AOF but not manufactured on AOF machinery. Express and Select are both a single recombinant enzyme."
Please let me know if this is not clear.
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