How much CO2 is needed for operation of 1 incubator maintained at 37 degrees C and 5% CO2 with normal activity (opening of door approx. 4 times a day) per week? Is there a calculator for this? The volume of the incubator is 250 L.
If it doesn't leak much the answer is 5% of 1000 L/d
Check on this publication.
https://www.eppendorf.com/product-media/doc/en/151861/Eppendorf_CO2-Incubators_White-Paper_029_CO2-Incubators_Best-Practices-Selection_Set-up-Care.pdf
these articles may help you:
Article A Regulated Incubator Controlling CO2 Concentration, Humidit...
Article Carbon isotopic composition of bacterial methane in a soil i...
Article Estimating soil labile organic carbon and potential turnover...
and also the file that i have attached
Son
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