No title

Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Any prompt reply would be highly appreciated Thank you,

09 October 2018 2,182 0 View

No title

Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Thank you,

09 October 2018 6,230 0 View

What is the required concentration of all three (Packaging, Envelop, and Transfer) vectors in Lentivirus Packaging?

I need to package lentivirus to generate gene of interest stable brain tumor cell line so, I want to know what is the required concentration of packaging, envelop and transfer vectors to transfect...

31 December 2017 2,649 2 View

How to interpret JC-10 Staining results

Hello Dear Researchers does anyone have experience to interpret JC-10 staining result for Mitochondria Membrane Potential. Could someone please explain me in detail with images because yet i don't...

11 December 2016 8,266 0 View

Manual Protein Extraction Protocol?

10 November 2016 8,427 2 View

What should be the minimum DMSO concentration to dissolve Temozolomide drug?

09 October 2016 2,937 3 View

Does anyone have exprerience with the situation when Insert and plasmid are not ligating?

I did the digestion of gene X and gene Y with respective enzyme and plasmid concentration and insert concentration and size everything is fine after digestion but after ligation I am not getting...

08 September 2013 8,036 2 View

Not getting desired band of inserts after digesting with respective enzymes?

I want to do subcloning of two genes (gene X and gene Y) in pcDNA 3.1+ expression vector. Gene X is having KpnI and NheI restriction site, gene Y having BamHI and NheI restriction sites....

08 September 2013 3,434 12 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View