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Questions related from Ruheena Javed
Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Thank you,
10 October 2018 6,384 0 View
Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Any prompt reply would be highly appreciated Thank you,
10 October 2018 2,303 0 View
I need to package lentivirus to generate gene of interest stable brain tumor cell line so, I want to know what is the required concentration of packaging, envelop and transfer vectors to transfect...
01 January 2018 2,751 2 View
Hello Dear Researchers does anyone have experience to interpret JC-10 staining result for Mitochondria Membrane Potential. Could someone please explain me in detail with images because yet i don't...
12 December 2016 8,369 0 View
Dear Researches, can some one provide me the Manual Protein Extraction protocol from Cultured cell?
11 November 2016 8,527 2 View
Dear researchers i want to treat U-251 cells with Temozolomide (TMZ) so, i want to know that what should be the minimum DMSO concentration to dissolve the Temozolomide and what should be minimum...
10 October 2016 3,067 3 View
what if re-autoclave PBS twice for Cell culture use? it will work normal or not?
10 October 2016 1,614 0 View
I want to do subcloning of two genes (gene X and gene Y) in pcDNA 3.1+ expression vector. Gene X is having KpnI and NheI restriction site, gene Y having BamHI and NheI restriction sites....
09 September 2013 3,528 12 View
I did the digestion of gene X and gene Y with respective enzyme and plasmid concentration and insert concentration and size everything is fine after digestion but after ligation I am not getting...
09 September 2013 8,136 2 View
