Ruheena Javed

9 Questions 6 Answers 0 Followers

Questions related from Ruheena Javed

Has someone experience in working with the "Adenosine 5¢-triphosphate (ATP) Bioluminescent Assay Kit" Sigma Aldrich?

Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Thank you,

10 October 2018 6,362 0 View

Has someone experience in working with the "Adenosine 5¢-triphosphate (ATP) Bioluminescent Assay Kit" Sigma Aldrich?

Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Any prompt reply would be highly appreciated Thank you,

10 October 2018 2,282 0 View

What is the required concentration of all three (Packaging, Envelop, and Transfer) vectors in Lentivirus Packaging?

I need to package lentivirus to generate gene of interest stable brain tumor cell line so, I want to know what is the required concentration of packaging, envelop and transfer vectors to transfect...

01 January 2018 2,735 2 View

How to interpret JC-10 Staining results

Hello Dear Researchers does anyone have experience to interpret JC-10 staining result for Mitochondria Membrane Potential. Could someone please explain me in detail with images because yet i don't...

12 December 2016 8,356 0 View

Manual Protein Extraction Protocol?

Dear Researches, can some one provide me the Manual Protein Extraction protocol from Cultured cell?

11 November 2016 8,515 2 View

What should be the minimum DMSO concentration to dissolve Temozolomide drug?

Dear researchers i want to treat U-251 cells with Temozolomide (TMZ) so, i want to know that what should be the minimum DMSO concentration to dissolve the Temozolomide and what should be minimum...

10 October 2016 3,046 3 View

What if re-autoclave PBS twice for Cell culture use?

what if re-autoclave PBS twice for Cell culture use? it will work normal or not?

10 October 2016 1,599 0 View

Not getting desired band of inserts after digesting with respective enzymes?

I want to do subcloning of two genes (gene X and gene Y) in pcDNA 3.1+ expression vector. Gene X is having KpnI and NheI restriction site, gene Y having BamHI and NheI restriction sites....

09 September 2013 3,516 12 View

Does anyone have exprerience with the situation when Insert and plasmid are not ligating?

I did the digestion of gene X and gene Y with respective enzyme and plasmid concentration and insert concentration and size everything is fine after digestion but after ligation I am not getting...

09 September 2013 8,122 2 View