Hello Sanchari Kundu

The dilutions of the standard (BSA) to be used in Bradford assay for the standard curve should be between 5μg/ml to 100μg/ml. At least five dilutions of BSA standard will be required for the standard curve.

So, in the method section, you may write as follows:

“The stock BSA standard solution was prepared at a concentration of 5mg/ml. The stock was pipetted into a set of tubes to achieve concentrations between 5 to 100 μg/ml of protein per tube.”

Hope this will help!

Best.

Thank you so much for your answer.

Hi Sanchari Kundu

Yes, it is common to report the concentration of the standards used in the Bradford assay in the methods or results section of a research paper. If your stock solution of BSA was 5 mg/ml, you could report it as such, or you could dilute it to create a standard curve with a range of concentrations (e.g. 0.1-1 mg/ml) and report the concentrations of the individual standards used. This information is important for the reproducibility of the assay and allows readers to compare the results of different experiments.