I am currently performing metabolic identification; however, the results tend to be quite subjective. In the assimilation tests, I even observe colorations that are neither indicated in the insert nor found in the literature.
Girma Mengesha Melese
Ethanol: Good for extracting a wide range of polar and non-polar compounds. Methanol: Effective for polar compounds and often used in extraction protocols. Acetone: Suitable for non-polar and semi-polar compounds. Chloroform: Ideal for non-polar metabolites. Water: Works well for polar metabolites but may not dissolve non-polar compounds effectively.
The choice of solvent for dissolving secondary metabolites extracted from fungi largely depends on the specific compounds you are targeting, as these metabolites can have varying polarities. Here are some common solvents used:
In many cases, a mixture of solvents (such as water and ethanol) can be used to optimize the extraction of diverse metabolites.
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