G'day Marcio --- What kind of resin? Assuming you mean a so-called water miscible resin, such as glycol methacrylate, the answer is you can do this. Although usually you will need to use a longer staining time than with a paraffin section. Same recipe other than that. However, as the resin itself does not stain, this is a mere inconvenience. See for some details an old paper of ours [Journal of Microscopy, Vol. 160, Pr 3, 1990, pp. 279-290. Staining sections of water-miscible resins. 1. Effects of the molecular size of the stain, and of resin cross-linking, on the staining of glycol methacrylate embedded tissues, P Gerrits, RW Horobin, DJ Wright]. If you cannot get hold of this, email me and I'll send you the pdf.

If your wanting to stain epoxy resin sections, well you still should be able to, although there will be problems. Let me know if this is what you want, and I'll put up some more infol

Bye --- Richard Horobin

Hi Dr. Richard. First of all, thanks for you help.

I'm not sure what type of resin the material is embedded (it has been in our lab for a certain time). The only thing I know is that it the tissue was fixed in buffered formalin and the brand name of the resin that it was used for the embedding: HistoResin kit by Leica. Any suggestions of it would still be possible?

Thanks in advance.

Marcio

HistoResin will be a "water miscible" material ... I put that in quotes since its the monomer which is water miscible, the resin itself will be slightly hydrophobic. ANYWAY this material should be stainable with Sirius Red, but as I said before you will probably have to stain longer than suggested in standard handbooks for paraffin sections. Oh, a thought: what is the nature of the biological sample in the block?

By the way, I see you're after another pdf of ours ... I will try to send it via ResearchGate. However, I have experienced some problems uploading file this way. So if the pdf doesnt turn up soon, email me on my home address [below] and I will send the pdf as an email attachment.

Bye --- Richard Horobin

... Oops! But I didnt add my home email! Oh dear ... OK it is:

[email protected]

Dr. Richard,

Thanks a lot for you help. I will send an email to you and we can keep in touch.

Regards,

Marcio.