What is the status of Genetically Modified Maize globally and nationally(INDIA). What is the recent qualitative improvement in GM CORN?

What is the status of Genetically Modified Maize globally and nationally(INDIA). What is the recent qualitative improvement in GM CORN?

04 May 2019 5,495 4 View

What are the reasons for PCR failure when DNA is extracted from degraded feces, conserved in 70% alcohol?

10 November 2014 4,623 17 View

What is the effect of the 260/280 value on PCR?

I know a value of 1.8 is the purest form of DNA.

10 November 2014 2,911 7 View

What should be done in order to conserve species from extinction?

If a species are endangered, such as Cervus elaphus hanglu and are only confined to a natural park, what steps should we take to conserve the species from extinction?

11 December 2013 6,064 27 View

How is the microsatellite characterization of DNA helpful for conservation of species?

Any comments?

10 November 2013 2,171 5 View

Is phenol-chloroform extraction best method for extracting DNA?

I think yes because we use an appropriate extraction buffer.

10 November 2013 1,979 2 View

What is the best method to extract dna from faecal matter?

Using the method using ezyna cycle pure kit, omega.

10 November 2013 5,791 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View