What pH buffer solution best replicates physiological conditions?

I am studying the interaction between protein (HSA) and liposomes (made with DMPC) and I would like to study under physiological pH for application in studying protein interaction with DDS. I have...

10 November 2020 3,594 3 View

All teacher educators are under the lockdown since March, what could they be doing, how is this lockdown going to affect tutors' performance ?

There are many COVID 19 related issues in terms of how to deal with the continuum of teaching and learning. I am meditating about what the future performance results will be for both the student...

16 June 2020 199 3 View

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03 December 2019 5,253 1 View

Reliable conjugated and unconjugated IL17-F anti-human antibody for flow cytometry?

Dear all, can someone kindly recommend a reliable fluorochrome-conjugated IL17-F anti-human antibody and also an unconjugated one please? I am studying CD3+ Lymphocytes from healthy volunteer...

19 February 2019 3,843 3 View

What is an acceptable sampling rate for skin conductance?

What is an acceptable sampling rate for measuring skin conductance? Research seems to use various rates, from 32Hz to 1000Hz. Is there a minimum rate advised? I have a device that measures at 4Hz...

03 February 2019 9,630 1 View

Is it possible to prepare SDS Reducing Buffer without including the bromophenol blue?

Hi all, I am doing an SDS-PAGE (Laemmli) gel electrophoresis but don't have any bromophenol blue to make up my SDS sample buffer. Is it possible to leave this out if you are confident of how...

26 January 2018 2,096 3 View

How to calculate the Feed Conversion Rate (FCR) in free-choice diets?

Hi! I would like to calculate the FCR for a diet-induced obesity experiment in rats. Since I have many nutrients/groups, I am calculating the food intake (FI) in kcal, not in grams of food. Has...

09 November 2017 5,258 5 View

What is the typical percentage error when corneal endothelial cells are counted using a specular microscopy ?

11 July 2016 7,906 2 View

How do I design Methylight primer/probes without using Beacon?

I want to Methylight assays and I need to be able to design the primer/probe sets, or get software that can do it for me. But I've only been able to find Beacon software that does both primers and...

08 June 2016 9,414 1 View

What Australian cities would be good to compare with Geelong, Australia in regard to economic, social & physical capital?

Newcastle and Wollongong are good examples but I am interested to learn about other large regional centres, particularly those that may have experienced a shift in their economy and have had to...

26 November 2015 5,759 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View