Has anyone used Dextran sulfate and sheared salmon sperm DNA as competitor in RNA aptamer selection? If yes, how are they helpful? Many research papers suggest use of this competitors, but does not really explain the reasoning behind using them.
Paul Rutland
I expect the dna is to stop non specific absorption of the more important reagents onto the plastic of the tube and possibly also to take out any moieties binding to common repeat sequencesand the dextran is used to effectively increase the concentration of the reagents so increasing the speed of reaction similar to its use in Southern blotting
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