You might try the Alexa Fluor analogs from molecular probes (Life Technologies)

Check out the DyLight products available on the Piercenet.com website.

There are some papers about the topic. I am also interesting in this field, since I have enough functional dye with the structure.

If possible, stay away from Cy3/Cy5 and the alternative dyes from Alexa, Dyomics, Dylight, Oyster and the like. They all belong to the same fluorophore class, which is photochemical unstable, prone to ozone bleaching and have a low quantum yield.

Labels from Atto-Tec or Seta Biomedical will be a much better choice...

I would suggest trying a spin-off company of my institute; Look at the spectrum; the dyes are more stable and have a higher wavelength fluorescence  http://www.biolabels.com/Oyster656-spectra.htm

thank you for the replies.. Right now, i have shifted to using fluorescent proteins.. will keep your comments in mind when I need to be using dyes.

I want to use cy3 or Dylight550 to label the total protein, who have the experience to label the total protein of tissue,

I can offer the labeling reagents of dyes cy3, cy5 and cy7.

Dear Vinod,

Since Cy dyes came from patent protection there are several suppliers of these dyes at reasonable, compared to GE. These are fairly simple compounds, but somehow tedious to purify, and often require several rounds of HPLC purification. I would not expect any low cost suppliers to be any different from GE. We offer both Cy3 NHS ester (https://clickchemistrytools.com/product/cy3-nhs-ester/) and Cy5 NHS Ester (https://clickchemistrytools.com/product/cy5-nhs-ester/  )

Hi,

You can buy it at Alfa Chemistry.

https://www.alfa-chemistry.com/search.aspx?q=Cyanine%20dye

I like to know what are FAX or fox dyes? and how they are used in assays?