Has anyone used clone B1 to block TCR of gamma/delta T cells?

More Sneha Sant's questions See All

Pls send me IEEE conference paper template if anyone have?

IEEE Conference template

02 August 2024 8,762 2 View

What are the environmental and health considerations associated with the production, use, and disposal of NiO nanoparticles?

24 April 2024 3,584 3 View

Hi all actually I'm working on finfet using SRAM so I have simulated the finfet part in LTspice is it possible to import that spice code into cadence?

I tried importing the spice code which I have simulated in LTspice into cadence virtuoso 45nm technology the following error is coming Did not find 'analogLib.PMOS:symbol'. ERROR (SPICEIN-24):...

15 April 2024 1,985 0 View

Name Suitable titles for Healthcare 4.0 using blockchain ?

I want to do research in Healthcare sector ..Suggest suitable titles for topic approval

15 August 2023 7,870 1 View

Mouse liver organoid poor growth after every passage and in the new stock. Why is that happening?

I am growing mouse liver orgnoids. I usually change the medium every 2 to 3 days. But after 2 passages, I can see the organoid growth is deteriorating. Even when I culture the new stock I cannot...

08 August 2023 4,986 2 View

What is the doubling time of T84 cell line? What should be the seeding density of T84 in a 96 well plate?

I seeded 8000 cells per well in 1 96 well plate. The number I think is very high for T84. So, I need the doubling time of T84.

06 June 2023 5,390 1 View

What is the protocol to dissolve chitosan in acetic acid?

I want to prepare chitosan solution for my work. I need a method to dissolve chitosan flakes in the acetic acid. Please suggest protocol for the same.

22 May 2023 3,836 5 View

New Development in Intrusion Detection System using machine learning approaceh?

I am searching for my phd Topic on Intrusion Detection system using machine learning approach.

04 May 2023 5,223 0 View

Alpha- amylase inhibition assay?

i am doing alpha- amylase inhibition assay for an in vitro gastrointestinal digest of an ayurvedic formulation- both pepsin digest and pancreatic digest. but for both of digest, amylase activity's...

18 April 2023 2,077 1 View

How to extract Moment (M) and Shear Force (V) for a Structural Wall from Etabs?

I have modelled a structural wall in ETABS using shell elements. I need to obtain the moment and shear force from it. How can we extract it from ETABS? Also, can anyone suggest any another...

29 January 2023 9,244 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Mengyong Yan

B1 clone is activating antibody rather than blocking antibody for gamma delta T cells. There are few blocking Abs for gamma delta T cells.

Sneha Sant

Agreed that it activates since it sits on the tcr but eventually it blocks any antigen mediated activation of tcr. It has been used as blocking ab in couple of papers.