Do you know any online database containing pictures (and explanation) about dry bone histology?

I'm interested in both pathological and normal histology.

15 January 2019 3,823 3 View

Why are E. coli strains used for overexpression of recombinant proteins often recA+ as opposed to recA-?

Many molecular biology E.coli strains are recA- to prevent recombination of the plasmid with the host chromosome, or scrambling of the plasmid, and/or mixing of more than one plasmid if...

09 May 2018 7,146 8 View

RNA isolation after TTC staining

How much the staining affects the RNA isolation and proteomic test after using the TTC stained heart tissue?

26 June 2017 9,924 2 View

No title

09 March 2017 3,472 3 View

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm. Can anyone help?

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm in my doctoral thesis. I would acknowledge and reference any provided SECM data. The...

30 May 2016 1,666 2 View

I need help converting a PAUP block into a MrBayes block - please help?

18 April 2016 8,561 1 View

Clinical Phase II to Phase III failures in oncology?

I am requesting the help of the RG community to provide me with recent (no older than 5 years) articles (from very high and only from very high ranked journals) clearly 1) indicating how many...

11 February 2016 4,471 18 View

How do you interpret a KEGG/KAAS output?

I've just run a KAAS analysis on my transcriptome. I'm a little unclear as to how to understand the output. For example, I have clicked on "Carbon Metabolism" and this image comes up (see...

21 April 2014 3,775 2 View

Increasing/decreasing fluorescence when measuring RNA concentration?

When we measured RNA concentration using fluorescence method with Modulus Turner Biosystems and measured the same sample a few times in a row without opening the lid, sometimes the fluorescence...

05 September 2013 2,591 5 View

Can petal colour be determined only by genetics (in Rubus: white or pink petals)?

01 January 1970 7,953 11 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View