Has anyone used Ampure XP beads to size select RNA without reverse transcribing it? Ideally I would like to isolate fragments 30-200bp from total RNA?
I have, for CEL-seq2 and also had the same question: https://www.neb.com/faqs/2014/08/15/can-i-use-agencourt-dna-ampure-xp-magnetic-beads-instead-of-rnaclean-xp-magnetic-beads
Peter, does it work?
Hello, I'm working on medical project and I would need synchronized angiography with ECG? Does anyone know if some open source dataset of this kind exist? Regards, Bruno
25 July 2024 2,214 2 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
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Hello all, I'm starting a project where we want to automate the video analysis of people working in different environments to produce ergonomic measures like hip flexion, shoulder extension, etc....
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dear all, I try to induce neuroinflammation in HMC3 cells using LPS for 24h. The concentration used was 500 ng/ml. But unfortunately no change on TNFa was obtained in LPS versus Control...
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I am searching for a validated measurement instrument to assess self-efficacy in health care professionals / medical doctors / general practitioners regarding their tasks in medical care and...
21 May 2024 656 0 View
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I'm performing an antibody phage display with a VHH library and I consistently get frameshift mutants (mainly frame +2) after biopanning. I'm using TG-1 cells for amplification of phagemids and...
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Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
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After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
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kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
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Molecular docking software/ websites?
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Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
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Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View
Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...
28 July 2024 2,136 4 View