Has anyone tried to add TMAO (or PEG20K) in gels for electrophoretic separation of proteins (non-denaturing conditions)?

04 April 2014 0 1K Report

The idea is to visualize high-order oligomers of a protein which can self-associate but normally exists as small oligomers. Is it OK to use native-PAGE and gels containing TMAO? Which TMAO concentration could be used without affecting normal electrophoretic separation of proteins?

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