Hello, I am a masters student and I am using the Sodium borohydride/Chloranil based assay for quantifying flavonoids in Eruca sativa. I am following the method attached by He, Liu, and Hai Liu (2008). I am using catechin-hydrate instead of catechin as my standard, but my standard curve turns out to be non-linear and my absorbances are low. I am also measuring the absorbances in a quartz cuvette by a spectrophotometer instead of a 96 well plate using a MRX Microplate reader. I read that results may vary with the use of these two different instruments. Is that the case or can it be something else?
Thank you,
Noor
Article Sodium Borohydride/Chloranil-Based Assay for Quantifying Tot...
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