Has anyone tried the EchoCLEAN TM CleanUp Kits from BioEcho Life Sciences for DNA cleanup? How is the efficiency like?

04 December 2018 0 9K Report

I've been having trouble with purifying my DNA post-PCR using gel extraction techniques as I lose all my DNA and have a 230nm peak which may be contamination by ethanol of guanidinium salts. Any suggestions for better methods? The EchoCLEAN kit supposedly would prevent this problem of contamination.

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Melissa Yeow's questions See All

How to convince the public to wear masks/gloves if they are resistant?

As you all know, this pandemic has taken a major toll on the entire global community. In some societies, people do abide by COVID protective measures. Yet, in other societies people are resistant...

30 September 2020 546 13 View

I am having a problem ligating my vector with PCR product by using the pcDNA3.1 V5-His-TOPO expression kit.Have anybody had such an experience?

I am trying to clone using the pcDNA3.1 V5-His-TOPO expression kit. There is no problem transferring it to the cell, but I can only transfer the vector. I did not succeed in vector-insert...

06 September 2020 3,483 0 View

What are these additional bands in my agarose gel results?

I have included pictures to illustrate my point. In the gel image that has only 4 lanes: Lane 1 is a 1 kb molecular weight ladder. Lane 3 is my wild type DNA (in a pGEX plasmid) and Lane 4 is the...

28 August 2020 4,146 4 View

Delta Furin SARS-COV-2 mutant Western Blot Analysis showed double band around 250 kDa. Processed and unprocessed version? Need evidence, please help?

I analysed SARS-CoV-2 Spike Protein delta Furin mutant in my bachelors thesis and need evidence (paper, research or something like that) to proof my suggestion. I think the double band at around...

26 August 2020 1,058 4 View

How to get SD from CI??

I apologize in advance for my rookie question. I have been looking in the internet how to get SD from CI values, and some sources tell me to apply the formula =SQRT(N)*(CI_upper.limit -...

19 August 2020 8,946 2 View

Who I should contact to get permission to use the BREQ-2 tool in academic research?

The BREQ-2 tool is commonly used in physical activity research to measure motivation. The tool was developed by David A. Markland. I have not been able to find how to ask consent for its use in...

15 April 2020 5,428 3 View

How to aggregate data from various surveys and reflect them onto one person?

So for example, we have the following polls: First poll: People who said they like orange juice for breakfast are as follows: Texas (50%), NY (25%), North Carolina (20%), and Florida (5%) Second...

01 March 2020 2,916 3 View

How do voter data companies collect or match info?

I have been recently studying proprietary voter files and data. While I know that voter files are for the most part public (paid or free), I am confused as to how companies match this data to...

30 January 2020 7,520 1 View

Is Bowtie2 assembly file saving problem caused by no space left on the disk?

Hello, Here is what I got with Bowtie2 while changing .sam file to .bam file: "samtools view -bS egpart1.sam > egpart1.bam [E::bgzf_flush] File write failed (wrong size) samtools view: writing to...

28 December 2019 3,244 2 View

What type of design?

I am asking for a student I'm mentoring. It's an action research project with a small sample of maybe 8-10 participants. If the student includes a likert attitude scale but is mostly focusing on...

08 June 2019 9,553 4 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View