Hugo Figueira de Paula Pinto

5 Questions 2 Answers 0 Followers

Questions related from Hugo Figueira de Paula Pinto

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

15 July 2024 6,227 0 View

Tol2 - Is it normal to lose the transgene expression after a few days?

I'm using a transposon tol2 vector (pT2AL200R150G - 10.1534/genetics.106.060244) to create transgenic zebrafish line. We first inserted our gene of interest (GOI) into the BamHI site of the...

22 November 2023 9,591 1 View

Has anyone tried Nightsea fluorescence adaptor?

We are exploring possibilities to adapt our stereo microscope due to the high cost of fluorescence stereomicroscopes. I recently came across this product...

17 May 2023 9,549 2 View

Why is takara infusion transformation protocol not working on large inserts?

I was able to transform bacteria sucessfully with small inserts (+-500bp and 1500bp) using infusion technic. However, when it comes to larger inserts (5500bp and 6000bp), it doesnt work. We...

06 December 2022 1,257 2 View

Can i insert the transposase (tol2 tpase) plasmid along with my transformed plasmid in zebrafish embryo?

Instead of inserting my transformed plasmids (my sequences of interest) with the transposase mRNA in zebrafish embryo, can I just insert both plasmids at the same time?

06 October 2022 8,577 1 View