What is the transfection efficiency in HepG2 with this reagent in your hands? ATCC claims that the transfection efficiency is about 95% in HepG2- this seems just a little too good to be true...
Thanks!
Well 95% efficiency is likely for siRNA transfection, and indeed in HepG2 it isn't that improbably (see this kit for example: https://altogen.com/product/hepg2-transfection-reagent-hepatocellular-carcinoma/). If you're transfecting plasmids into your cells, then obviously the efficiency will be lower, but generally speaking HepG2 is easily transfectable.
we just used in stem cell a year before. seem good. we just used lip2000 for cancer cell lines, it' s enough for transfecting
Dear Sunnie,
I would like to propose you an alternative to "classical" transfection reagents : the Magnetofection*** transfection method.
very briefly, after complexion to nucleic acids, magnetic vectors are attracted onto the cell surface by a specific magnetic field that enhances the overall efficiency. This method was demosntrated to be highly efficient in HepG2 by many authors using any kind of nucleic acids:
- DNA (PolyMag/Magnetofection): Montrose K., Biochem Biophys Res Commun. 2014.
- miRNA (Magnetofection): Dang Y., PLoS One. 2013.
- siRNA (SilenceMag/Magnetofection): Chen J., BMC Cancer. 2014
for a complete list of publications about HepG2 cell transfection, you can refer to: http://www.ozbiosciences.com/module/citationfinder/default
would you have any question please do not hesitate to contact me via ResearchGate or directly at: [email protected]
good luck with your experiment,
best regards,
Cedric
*** The Magnetofection technology uses a magnetic field to attract and concentrate complexes of magnetic nanoparticles and nucleic acid onto the cell surface as demonstrated by Grześkowiak BF et al, Pharm Res. 2014 Jul 18.
http://www.ncbi.nlm.nih.gov/pubmed/23593387
http://www.ncbi.nlm.nih.gov/pubmed/24555445
http://www.ncbi.nlm.nih.gov/pubmed/25305486
Zhongliang and Cedric, thank you for your input! I have used magnetofection to improve the transduction efficiency in a cell line, but that was a long time ago- thanks for jogging my memory!
Dear Sunnie,
you are welcome, I will be glad to send you some samples if you want to use magnetofection again, just tell me :)
best regards,
Cedric
Well 95% efficiency is likely for siRNA transfection, and indeed in HepG2 it isn't that improbably (see this kit for example: https://altogen.com/product/hepg2-transfection-reagent-hepatocellular-carcinoma/). If you're transfecting plasmids into your cells, then obviously the efficiency will be lower, but generally speaking HepG2 is easily transfectable.
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