If yes, could I please get some helpful tips. I am trying with Lipofectamin 2000 and have had no success as yet. It will be great to know if someone has been successfull.
Thanks
Sindhu
Dear Sindhu:
I do not know your lab economical situation. But if you consider the cost of producing the transfection vs the cost of buying those cells from ATCC I think it will be cheaper to buy them and they will be certified also. Check: https://www.atcc.org/Products/All/CRL-4031.aspx
Best of lack
Daniel
Apologies if I was not clear in my question. I already have this cell line. We bought them from Evercyte. I am not trying to transfect them to create stable KO cell line using Lipofectamin. I will be transfecting them with CRISPR plasmids.
Hello, we have 2 different customers who were quite succesfull with both Viromer RED and Viromer YELLOW (60-70%). But with some toxicity, medium needs to be changed after transfection. Free test samples are available at http://www.bioscience.co.uk/cpl/135754/
Hi Sandra, Is it possible for you to let me know the conditions with which the customers got good efficiency with the transfection? I cannot find this information on the website as the list of cell types successfully transfected does not include RPTEC/TERT1 cells yet. Thanks a lot.
yes, it's not on the website because it's confidential data. But as I said before, the only notable thing is the change of medium to avoid toxicity issue. For the rest, the standard conditions work well. Best, Sandra
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