Has anyone successfully expressed and purified a trimeric protein complex in mammalian tissue culture cells for analysis via crystalization?

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Stefan Gajewski

maybe someone has done that before.

However, you want to express your proteins for crystallography in bacteria unless you have a very rich aunt who likes you a lot. :D

seriously, make the protein in bacteria and reconstitute the complex in-vitro. You are interested in ~50-2000uM highly pure sample when trying to grow crystals.

Dan Ryder

Lol! Yes you have a good point. Thank you Stefan.