the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

Can you help with the implementation of two different model of deep learning for stock market prediction?

I have stock price data(Open, high, low, close), I want to do prediction by combining two different models like CNN with others. Dataset will be same, what are the ways that I can use for...

04 January 2021 1,845 3 View

If we forget to add mercaptoethanol, can we correct the samples somehow?

Hi everyone, Can someone help me with a western blotting troubleshooting? So during sample prep, we boil the samples with 2-mercaptoethanol in Laemli buffer. However by mistake, some of the...

02 December 2020 6,859 3 View

How to isolate human cardiac stem cells? Which other cell types could be in this cell culture?

Hello, I followed a protocol for isolating human cardiac stem cells (Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human...

18 November 2020 2,928 3 View

Please where can i buy pure Nitrogen gas in Nigeria?

I need just a very small quantity of pure Nitrogen gas for use in my locally designed anaerobic glove box. Any information would be of immerse help to my research work. Thanks

11 October 2017 2,853 6 View

How can I create the .nk files of materials in Silvaco atlas?

I'm a simulating a solar cell which includes the material GaAsP. The .nk file of GaAsP is not available in the sopra database..if i have the n,k data of this material can anyone tell me how can i...

21 March 2016 1,906 8 View

How do I upscale data from farm scale to village scale and to regional scale?

I am going to prepare a survey for farmers in East Africa. The data aquired I want to upscale to village scale and from there to regional scale. I am searching established methods for this...

14 December 2015 9,083 3 View

Does anybody know about an anti-mouse CD11d antibody that works in FACS?

Thanks in advance for your answers.

24 March 2014 659 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View