cDNA is DNA, and it is fairly stable than RNA, I would try to do PCR and see what happens before doing fresh cDNA.

It should be fine, as long as it not left in the hood with UV on :)

Shouldn't be degraded, you could probably leave it for months and it would still be fine.

DNA is absolutely stable, otherwise it would not be the possible to extract DNA from the samples that is several thousand years old ! Especially if u used elution buffer, which perfectly keeps a DNA.

Overnight it should be fine, as long as the cDNA is pure enough, without other contaminants which may degrade them at room temperature.

Totaly agree, only one night should be OK.

cDNA is very stable if you had diluted it with DEPC water for your quantitative RT-PCR

I agree with all, your cDNA should be fine for further qPCR analysis.

No problem you can go ahead with that cDNA, cDNA is stable for months.

I also agree, cDNA is very stable. It should still be perfectly fine for qPCR

I made the same blunder. Poured out my ice bucket forgetting to place tubes in fridge. -_-

Edit. Samples were okay.

Hi everyone,

I am also unfortunately forgotten the samples on the PCR device for 4 days! The temperature at some of the days in the room reached 45 degrees Celsius (there were hot days)

My examples are still fine?