Varun Chandrashekaran

3 Questions 10 Answers 0 Followers

Questions related from Varun Chandrashekaran

Why are all my protein bands accumulating in the lower half of the gel?

Dear all, I ran my protein samples on a 8% polyacrylamide gel at 110v for about an hour. Interestingly all my protein bands accumulated in the lower half of the gel. After transferring the...

02 February 2018 759 0 View

Has anyone performed real time PCR with cDNA that was accidentally left out overnight.?

I accidentally left my cDNA outside at RT overnight, I ran the GAPDH and it seems to amplify correctly. However I am concerned about the low abundance genes, which may have degraded. Does anyone...

11 November 2014 3,665 12 View

Does anyone have any experience with transfecting two miRNA's, siRNA's into cells using RNAimax?

I search of a protocol for co-transfection of two miRNAs into HCT-116 cells using RNAimax.

09 September 2014 9,677 4 View