Model specification for moderation in multilevel model with R?

I've got longitudinal data from a psychological experiment and try to model the data using R package lme4. My dependent variable y is continuous and is measured at eleven occassions. Each...

13 April 2020 6,619 4 View

How to connect hypothesis to your discussion?

Hello, The question was answered. Thank you! Kind regards

12 January 2020 1,834 2 View

Convert units of pre-exponential factor in Arrhenius equation for reaction rate?

I found several pre-exponential factors for the reaction rate of calcium carbonate crystallization. They are usually given in m^4 kg^-1 s^-1. Is there any change I can convert them to m^4 mol^-1...

08 October 2019 3,770 1 View

Is it true that light produced in the core of the sun needs a million years to reach the surface?

Atoms in the sun core are very fast moving. I read, that for this reason the light produced as a by-product of hydrogen fusion cannot pass directly to the sun surface (which would be a matter of...

19 January 2019 3,495 3 View

If we look from Earth into deep space with our telescopes beyond our galaxy, is the universe uniformly dense in all directions?

26 January 2018 2,314 5 View

Best way to clean CTFE equipment in order to avoid DNA contamination?

Dear colleagues, I received different pieces of lab equipment (mainly pestils and alike) out of CTFE and would like to reuse the same pestils without autoclaving them every time. I do not have any...

29 August 2017 4,356 2 View

What shall I do, when I conducted an exploratory factor analysis and it doesn't identify two of my constructs as separated constructs?

I conducted an explanatory factor analysis (I used different rotations and used principal component). The explanatory factor analysis wasn't able to identify two of my model's constructs as...

25 July 2017 8,445 2 View

(Spatial) Working memory task in headfixed mice ?

I am investigating (spatial) working memory in mice. Due to the nature of the project the mice are head-fixed and therefore are not able to move around freely, eliminating most common tests for...

04 July 2016 2,997 2 View

What are best practices and relevant tools of sustainable financial investments (& insurance meachnisms) in rural activities and/or landscapes?

In many rural areas of "developing countries" small scale agriculturalists (farmers/fishers/herders/...) see the necessity for (financial) investments, they have the capacities (social & human...

25 June 2015 5,367 5 View

Is there a metal ion free developer that has a reduced aluminum etch rate?

We want to perform a lift-off on top of a nanometer sized aluminum layer, without having most of the Al removed during development of the photoresist pattern.

13 May 2014 5,257 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View

How seniors face covid 19 without fear?

Seniors in the world with diabetics and hypertension and heart diseases are millions ,and they are facing covid.therefore we should give them advices and precausions to face covid 19,this...

26 January 2021 8,349 4 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View