Dear all,

At the moment, I am planning bone marrow transplantations with different bone marrow subsets into lethally irradiated mice.

Since I am very unfamiliar with the topic, I ended up with several questions.

If I inject only HSCs (LSK+ CD150+ CD48- CD135-) into lethally irradiated mice, what chimerism efficiency can I expect in the peripheral blood? 50-60%? Where do the other (non-donor) cells derive from?

And what is the rationale behind using "helper marrow cells" and what exactly are they comprised of?

Thank you all for your answers and help!

Best wishes,

Max