Has anyone observed an red lagging band while running gels for SDS-Page?

Christopher Walker @Christopher-Walker-38

06 September 2019 0 9K Report

During electrophoresis, I observed a lagging read band on my gel. I have done many Westerns and have never encountered this before. I spoke with my PI and some others and they haven't observed it either. The only difference between this run and what I have done in the past, is the use of a 4x LDS Sample Buffer from a manufacture that we do not typically use. I contacted the manufacturer and was told that this is most likely from an issue with their product.

Has anyone encountered this before? If so, how did it alter your transfer and quantification?

This will not be used for publication. However, I would like to know if it would be possible to continue on so I can get some preliminary data. I hate the idea of wasting so much tissue as I had prepped lysates for several gels.

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