I am planning to use single nuclei sequencing to characterize an iPSC neuron/astrocyte co-culture model. I am setting up cultures to try and optimize the number of input wells, buffering conditions, cell dissociation conditions, and nuclei isolation conditions, but I have had very little luck in finding any kind of starting protocols, particularly for nuclei isolation. The majority of what I have found is specific for tissues, not cells. Right now, I am planning on working off the 10X genomic protocol, but I want to know if someone has worked on this method specifically with cultured iPSC neurons, since they tend to behave much differently than cultured cell lines.
Any suggestions on where to begin are appreciated!
You can use following kit:
https://inventbiotech.com/products/minute%E2%84%A2-single-nucleus-isolation-kit-for-tissues-cells-20-preps?_pos=2&_sid=48dff6ceb&_ss=r
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