We tried working with FTA kit, but it didn't seem to work for us for extracting DNA from Commiphora wightii and Prosopis cineraria plant species.
The Whatman FTA kit works best for extracting DNA from FTA filter paper.
We are working with a comparative functional genomics approach, where we are trying to make primers for a sequenced organism, but using these primers to pick-up genes in a related genus (but same...
02 March 2013 8,206 7 View
Is there a way to remove EtBr from solutions and then drain the 'cleansed' solution down the drain?
01 February 2013 9,760 13 View
Also, is there a non-spectrophotometeric way to determine purity of total RNA? I am working with plant samples.
01 February 2013 7,007 3 View
How many trees have their whole genome sequenced?
01 February 2013 2,578 4 View
My lab presently has an Alpha Innotech gel imaging system with proprietary software. I want an open software that can be used for analysis of the gel images on other computers and is possibly...
01 February 2013 5,467 4 View
Using Arabidopsis as the baseline whose genomic information is available and a crucifer close relative whose transcriptome is to be analyzed
31 December 2012 2,989 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View