Any tips for cross-species hetrologous transcriptome genomics / gene specific reverse transcriptase-PCR based semi-quantitative gene expression study?

01 January 2013 0 3K Report

Using Arabidopsis as the baseline whose genomic information is available and a crucifer close relative whose transcriptome is to be analyzed

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What could be reasons for mismatched amplicon sequence, even when primer blast picks the right sequence?

We are working with a comparative functional genomics approach, where we are trying to make primers for a sequenced organism, but using these primers to pick-up genes in a related genus (but same...

02 March 2013 8,122 7 View

Has anyone had a good experience working with Whatman FTA kit for plant DNA or RNA extractions?

We tried working with FTA kit, but it didn't seem to work for us for extracting DNA from Commiphora wightii and Prosopis cineraria plant species.

02 March 2013 8,736 1 View

What is the best way to dispose of ethidium bromide solutions and gels?

Is there a way to remove EtBr from solutions and then drain the 'cleansed' solution down the drain?

01 February 2013 9,673 13 View

Method for quantification of RNA without using any sort of spectrophotometer? Does it apply for DNA samples also?

Also, is there a non-spectrophotometeric way to determine purity of total RNA? I am working with plant samples.

01 February 2013 6,932 3 View

Have all tree species been completely sequenced? And how many tree species have their genomes sequenced?

How many trees have their whole genome sequenced?

01 February 2013 2,500 4 View

Please suggest one good free and open source software (FOSS) that can be used with any gel-documentation system for gel image analysis

My lab presently has an Alpha Innotech gel imaging system with proprietary software. I want an open software that can be used for analysis of the gel images on other computers and is possibly...

01 February 2013 5,373 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View