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I am trying to get a duplex qPCR method to work. When running the two reactions (Gene, and ACTB-ref) in singleplex, the gene gives me a perfect sigmoid curve. The ACTB has a tendency to be "flat"....
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I am trying to compare array snp calls to ngs snp calls, by using the position of the genotype. However the builds used differ, so I would like to make a liftover of the array data to meet the ngs...
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