Tarun Kant

7 Questions 16 Answers 0 Followers

Questions related from Tarun Kant

Has anyone had a good experience working with Whatman FTA kit for plant DNA or RNA extractions?

We tried working with FTA kit, but it didn't seem to work for us for extracting DNA from Commiphora wightii and Prosopis cineraria plant species.

03 March 2013 8,779 1 View

What could be reasons for mismatched amplicon sequence, even when primer blast picks the right sequence?

We are working with a comparative functional genomics approach, where we are trying to make primers for a sequenced organism, but using these primers to pick-up genes in a related genus (but same...

03 March 2013 8,168 7 View

Method for quantification of RNA without using any sort of spectrophotometer? Does it apply for DNA samples also?

Also, is there a non-spectrophotometeric way to determine purity of total RNA? I am working with plant samples.

02 February 2013 6,975 3 View

Please suggest one good free and open source software (FOSS) that can be used with any gel-documentation system for gel image analysis

My lab presently has an Alpha Innotech gel imaging system with proprietary software. I want an open software that can be used for analysis of the gel images on other computers and is possibly...

02 February 2013 5,427 4 View

Have all tree species been completely sequenced? And how many tree species have their genomes sequenced?

How many trees have their whole genome sequenced?

02 February 2013 2,540 4 View

What is the best way to dispose of ethidium bromide solutions and gels?

Is there a way to remove EtBr from solutions and then drain the 'cleansed' solution down the drain?

02 February 2013 9,720 13 View

Any tips for cross-species hetrologous transcriptome genomics / gene specific reverse transcriptase-PCR based semi-quantitative gene expression study?

Using Arabidopsis as the baseline whose genomic information is available and a crucifer close relative whose transcriptome is to be analyzed

01 January 2013 2,959 0 View