Has anyone experiment work with mirdeep software?

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Anita Tripathi

there's no need to install mirdeep u have to just download it and only have to run the scripts present in it but, before running mirdeep, you must have installed

LINUX OS, Perl, Bowtie and RNAfold....

Mohsen Honarpisheh

Hi Anita

I downloaded all but I do no know how insert sequence to this software becuse this software based on linux and perl and after run the program i got only command line in mideep do you know how does it work?

David Langenberger

Check out http://www.nature.com/natureevents/science/events/47533-microRNA_Analysis_Using_Next_Generation_Sequencing

Ellango Ramasamy

Hi Mohsen,

I recommend Biolinux-8 OS which includes all necessary packages and dependences for NGS data analysis (miRDeep2). follow the instruction (commands) and install it. Then prepare your data set (transcriptome reads, reference genome etc) as per guided in mirdeep2 webpage and do the sample run using C.elegans data provided in tutorial.