I have done sequential extract with pet ether, ethyl acetate and methanol. Now getting high antioxidant activity in ethyl acetate extract than methanol. I dint find any literature for this. Is there any particular reason for this?
Dear Mr Rajesh, as far as the antioxidant activity is concern, it mainly depends upon the phenolic and flavonoids constituents of the sample. You could quantify the phenolic, flavonoids and some other phytoconstituents which are responsible for the free radical scavenging activity and if there will more phytoconstituents are present in the ethyl acetate extract than methanol it will be clear that the free radical scavenging activity will be higher in the ethyl acetate extract.
Dear Rakesh, I am in agreement with Dr. Devesh's comment, but as far as any biological activity is concerned the solvent in which most of the phytoconstituents are extracted will show best activity, so it is not necessary that always methanolic extract will show better antioxidant activity than ethyl acetate. If you have observed better activity in ethyl acetate extract, it might be possible because the phytocompounds are better extracted in in ethyl acetate in your case.
I agree with the previous answer. Furthermore, When you say more activity what do you mean? You should use specific activity to determine whic extract is better e.g. Measure of activity/ amount of substance in extract. It may also depend on what you want to do with the extracts. For example, if you are aiming to purify a compound you might find an extract with lower activity may be more pure than one with higher activity (as it may have extracted lots of other materials as well).
it happens many a times coz ethyl acetate is a solvent which can efficiently solubilize flavonoids which are polyphenolic compounds. you can refer my paper https://www.researchgate.net/publication/234060667_Antioxidant_Activity_of_Callus_Culture_of_Vigna_unguiculata_%28L.%29_Walp
Article Antioxidant Activity of Callus Culture of Vigna unguiculata (L.) Walp.
If you perform a sequential extraction with petroleum ether, ethylacetate and metanol most antioxidant components will be present in the ethyl acetate fraction. The most important phytochemicals are phenolics and carotenoids, which both are extracted to a large extent with ethyl acetate. If you would change the extraction order by first extracting with methanol and then with ethyl acetate, I expect that most antioxidant activity will be present in the methanol extract.
Mr. Koen Goiris I have performed sequential extraction starting with petroleum ether, ethyl acetate and then with methanol. but in my previous works i got more antioxidant activity with methanolic extract but in this time i got in ethyl acetate extract. i refered some article all of them have more antioxidant activity with methanolic extract. can you help me with article having high antioxidant activity in ethyl acetate extract.
Dear Mr Rajesh this paper my be give you some informations
Article Phenolic profiles and antioxidative effects of hawthorn cell...
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