Has anyone experience with fixable viability staining of bacterial cells?

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[ Call for Papers ] INTELLIGENT TRANSPORT SYSTEMS: ECOLOGY, SAFETY, QUALITY, COMFORT ?

DEAR COLLEAGUES!We invite you to take part in the InternationalConference "Intelligent Transport Systems:Ecology, Safety, Quality, Comfort", which will beheld on November 26–27, 2024 Available...

10 July 2024 8,173 3 View

Can electron pairing in superconductors be derived from the presence of real-space-states of electrons?

Every crystal contains a set of spatial ground states, which may be occupied by electrons with opposite spins, forming singlet pairs. Every pair breaking event means that one of electrons leaves...

16 January 2024 9,253 10 View

Will a persistent supercurrent in a superconducting (SC) ring decay, if we connect the SC ring to a wire and the remote end of the wire is not SC?

The seemingly simple question, but nobody can answer it unambiguously. Experimental setup to the question is shown in Figure 1 in Tuning of Lifetime of Cooper pairs in a Massive Aluminum Ring A...

14 September 2023 8,683 5 View

Sanger sequencing problems: chromatogram quality inconsistency?

Hello, I have problems with plasmid and PCR fragments sequencing (Sanger sequencing on SeqStudio device). We perform sequencing reaction with BrilliantDye™ Terminator v3.1 with subsequent...

11 December 2022 8,197 3 View

Who can recommend the available methods or data for estimating resource intensity (f.e. Carbon Foot Print) for the Technology Assessment?

How to estimate the green (carbon) impact of the new technology/material for the infrastructure construction? This is important for forecasting changes in the cost of materials and assets, taking...

03 October 2020 9,812 5 View

What are the ways to sum two study results in one 2D Plot in Comsol?

I'm using the Comsol 5.5 to simulate the distribution of potential V along the length of the line (transmission line). Frequency domain analysis. I use 2 study - the first 10 Hz, 1 volt on the...

01 July 2020 9,728 3 View

How is COVID-19 impacting your own research?

Dear fellow researchers, Mohammad Soliman and I make a survey on the impact of COVID-19 on research. I kindly ask you to complete this questionnaire: http://bit.ly/Covid19Research Please share...

22 March 2020 2,268 11 View

Is the second quantization notation applicable for crystals, where identical valence electrons occupy both local and nonlocal states ?

The second quantization notation operates with occupation numbers. For electrons within a quantum system the occupation number of each state is 1 or 0. However, local states in crystals do not...

09 March 2020 5,242 14 View

Insect culture media compatible with PEI transfection of HighFive cells?

I gonna try PEI-based transient gene expression in insect cells (High Five), the protocol described in: A simple plasmid-based transient gene expression method usin... Authors successfully used...

24 October 2018 8,945 2 View

How to compare two waves of cross sectional data sampled from the same population; cases are unmatched?

I have two datasets in two files: a. survey data from wave 1 in 2010 2 b. survey data from wave 2 in 2015. The survey questions are unchanged. The two samples are not matched though are from the...

05 December 2017 9,443 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View