I work with zebrafish embryos, and I want to analyze open chromatin domains (via ATACseq) of a cell population. The only way to isolate this population is through laser capture microdissection (LCM). I have already looked through the literature and other forums for guidance on how to acquire good samples for ATACseq, but have not found any examples of ATACseq being done on LCM tissues. Has anyone on ResearchGate attempted ATACseq from laser capture tissues, and if so, how was your tissue prepped? Were there any major issues you had to address to get good quality data?