Visualizing extracellular matrix of cells?

14 February 2022 0 320 Report

Hello,

I would like to visualize the extracellular matrix of breast cancer cell lines MCF-7 and MDA-MB-231

In our facility we have Confocal microscopy. Anyone has any idea of how to perform that? I am planning to compare before and after treatment effect on ECM

Thanks!

Badges
Science topic

More Krista Mf's questions See All

Problem with relaxation?

Hi all, Anyone can help me to fix the problem, VERY BAD NEWS! internal error in subroutine IBZKPT: Reciprocal lattice and k-lattice belong to different class of lattices. Often results are still...

20 August 2023 8,107 2 View

No Amplification detected in RT-PCR?

Hello, I performed RNA Extraction from ~90 blood samples by TRIzol, got a good RNA and converted it to cDNA, then diluted all the cDNA to 500ng/ul for RT-PCR. I performed GAPDH first, the ct...

20 June 2023 9,581 3 View

Facing problem related to POTCAR??

My Calculation run in VASP, and the calculation has a problem. So, how I can solve this problem, please?

10 April 2023 5,914 7 View

Why am I getting second peak in RNA measurement?

Hello colleagues! I performed RNA extraction for my cells using TRIzol, and when I measured the yield using nanodrop, I got two peaks of RNA. I read that it is because of phenol contamination....

16 April 2022 9,316 3 View

Why am I getting horizontal running lines on my western blot gel?

Hello everyone, I was running my 10% gel, I ran it on 90V for 30 minutes followed by 120V for around 1:20, then used semi-dry transfer to nitrocellulose membrane. When I developed my blot, I got...

26 February 2022 5,415 0 View

Does anyone have recommendation on survey scanning software or service?

We are going to be collecting anonymous data from about 5K-6K active duty soldiers in person, about 200 at a time. Email is not an option. We had originally planned to use Scantron software and...

08 September 2019 715 0 View

Why when I group all my 10 constructs my hypothesis are rejected?

When I test the constructs by parts the hypotheses are confirmed, when together with the others, several hypotheses are rejected. Is there anything that can be done? In addition, the indices are...

19 July 2019 1,581 3 View

Confusion Matrix vs. ROC Curve in ENVI 5.3 to evaluate a classifier?

Hello all. My question is quite similar to one posted by Ahmed Ibrahim in March 2016 ("ROC vs Confusion Matrix for classifier evaluation?"), but I was hoping to ask a couple additional questions...

16 January 2018 8,140 6 View

Does anyone have a working link to the FG-NET ageing database or any other ageing database ?

I have been trying to find on a online source for FG-Net aeging database , MOPRH database and YGA database. I can't seem to find none of them available to download . Does anyone know any other...

20 February 2017 2,843 5 View

Has anyone ever tried ATACseq on laser captured tissue?

I work with zebrafish embryos, and I want to analyze open chromatin domains (via ATACseq) of a cell population. The only way to isolate this population is through laser capture microdissection...

03 November 2016 949 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...

08 August 2024 6,690 1 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Request a single Lecture notes for math as detailed as this that I can find in one place?

- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...

03 August 2024 2,366 0 View