Has anyone encountered such a problem?

More Yin-biao Wang's questions See All

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

No title

How can I know the expression level of common tumor targeting receptors on cancer cell lines? For example the cell lines and receptors I mentioned in the question. Thanks a lot.

31 August 2020 5,347 3 View

How can I get H-bond versus time graph and hydrophobic residue distance versus time graph from Gromacs ?

I am using Gromacs to analyze the interactions between protein-ligand.I am a beginner of Gromacs simulation. I have finished the simulation, I got the final gro , xtc, cpt, edr files, but not...

05 August 2020 3,401 3 View

How to make parrallel inlet boundary UDF work?

Hi everyone, It is quite urgent for me! I am doing 2D rotating detonation engine (RDE) simulation in ANSYS Fluent. For inlet boundary condition, there are three possible cases: 1. Pi > Po,...

02 August 2020 8,443 1 View

Preparation of PVDF Piezoelectric Sensor?

My main research is electrospinning of PVDF as piezoelectric sensors. According to the description in papers, I "Used PI/PET film as the substrate, pasted conductive tape as one side electrode,...

19 July 2020 5,287 2 View

2D rotating detonation simulation - How to implement ANSYS UDF boundary condition?

Hi everyone, I am doing 2D rotating detonation engine (RDE) simulation in ANSYS Fluent. For pressure inlet boundary condition, there are three possible cases: 1. Pi > Po, where Pi is the...

25 May 2020 7,040 16 View

The collagen gel evaporate with 3D organoid model (air Liquid interface method), why?

Hi, guys, I have a queation, please help me. I used air liquid interface method to do 3D culture. This is the first time to do it. the collagen gel prepared according to literature, 8 vol of...

13 May 2020 7,288 2 View

Why OCP is differnet when test the same BFC with fruits and animals?

I developed a BFC (GOD anode and BOD cathode), tested it with apple and mouse's blood, the OCP with apple was 0.62V, but the OCP with mouse's blood was only 0.5V, I thought the OCP should be same...

25 March 2020 2,866 1 View

How do we judge the mediation hypothesis was supported or not if BC interval confidence did not include zero, but estimate is small?

Hi, I'm a postgraudate student and thanks all in advance for kindly giving me some suggestions and comments. I'm testing a model with multiple independent variables and mediators using...

17 December 2019 8,994 6 View

Doing factor analysis in SPSS, what if the reproduced correlation saying that there are 75 (15%) nonredundant residuals with absolute value >0.05?

I'm doing factor anlaysis in SPSS and the reproduced correlation saying that the residuals are computed between observed and reproduced correlations that there are 75 (15%) nonredundant residuals...

22 October 2019 1,029 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Alexander N Volkov

HIs tag has perturbed the paratope? The E.coli expressed protein lacks the native post-translational modifications?

Yin-biao Wang

Maybe. Yet the target protein is reported to contains 5 to 8 dominant epitopes. It seems that no epitope would react with the positive serum. I'm going to find more sera to test or to express a truncated one of the protein. Anyway, thank you for you answer. Alexander