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Questions related from Yin-biao Wang
I need to use viral antigen from enveloped virus as the coated antigen in ELISA. should I extract these viral antigen from the cell culture supernatant or use the entire cell culture? Any...
12 December 2014 4,844 3 View
Dear All, A large amount of soluble protein of my interest could be harvested in E. coli. Yet, the protein seems to have rather low binding capacity with Ni-NTA column. The addition of 20 mM...
07 July 2014 8,011 4 View
Eukaryotic expression of foreign gene is performed in many labs. Could we fix target proteins on the surface of cells through some strategies instead of expressing it in the cytoplasm or...
09 September 2013 3,936 4 View
The His-fused viral protein expressed by E. coli only reacts with anti-His6 monoclonal antibodies, but fails to react with positive serum against the virus.
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