The His-fused viral protein expressed by E. coli only reacts with anti-His6 monoclonal antibodies, but fails to react with positive serum against the virus.
Maybe. Yet the target protein is reported to contains 5 to 8 dominant epitopes. It seems that no epitope would react with the positive serum. I'm going to find more sera to test or to express a truncated one of the protein. Anyway, thank you for you answer. Alexander