Yin-biao Wang

3 Questions 3 Answers 0 Followers

Questions related from Yin-biao Wang

Can anyone give me any methods for preparing viral antigens (e.g. from enveloped virus) to be used in ELISA? Any papers?

I need to use viral antigen from enveloped virus as the coated antigen in ELISA. should I extract these viral antigen from the cell culture supernatant or use the entire cell culture? Any...

01 December 2014 6,854 3 View

Can anyone help me with Ni-NTA affinity purification?

Dear All, A large amount of soluble protein of my interest could be harvested in E. coli. Yet, the protein seems to have rather low binding capacity with Ni-NTA column. The addition of 20 mM...

07 July 2014 6,153 3 View

Has anyone encountered such a problem?

The His-fused viral protein expressed by E. coli only reacts with anti-His6 monoclonal antibodies, but fails to react with positive serum against the virus.

17 July 2013 6,352 2 View