Has anyone encountered interference of Fe3O4 with tetrazolium salts-based viability assays?

More Roxana Cristina Popescu's questions See All

Can ROBINS-I be used to measure risk of bias in single-arm studies?

I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...

14 July 2024 3,570 5 View

What are these aggregates in activated sludge flocs?

I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...

23 June 2024 9,584 1 View

Extraction of intracellular protein with TRI reagent for ELISA?

I use ELISAs for quantification of extracellular proteins in cell-conditioned media. I am interested in quantifying uncoupling protein 1 (UCP1) which is an intracellular, mitochondrial...

27 May 2024 8,025 1 View

Can Sensory marketing affects customers satisfaction in Hotels?

12 May 2024 3,308 3 View

Is there a simple formula to estimate the prevalence of a disease (in this case, carriage) in a longitudinal one-stage cluster sampling?

Hello everyone, I would greatly appreciate it if someone could assist me in understanding the steps required to estimate the sample size for a longitudinal study. Or advise bibliography. I am...

04 April 2024 2,882 0 View

Should I block compensation beads with mouse serum in flow cytometry?

I am using mouse serum to block Fc receptors before staining, for flow cytometry. My question is if I should also add the serum to compensation beads before staining them, so that the cells and...

07 March 2024 9,989 2 View

Can staining for flow cytometry be done in flat bottom 96-wells plates?

Most staining protocols for flow cytometry in 96-well plates use V-shape or U-shape plates. I would like to ask if staining could also be done in flat-bottom plates. Thank you!

07 January 2024 2,910 5 View

Do FMO controls for flow cytometry need to be run in each experiment?

I plan to run my experimental samples in the flow cytometer in batches. My question is if FMO controls have to be prepared and run every time I run experimental samples, or if I could only do them...

04 January 2024 7,842 0 View

Statistical analysis for PBMCs immunophenotyping by flow cytometry?

I am immunophenotyping PBMCs by flow cytometry using markers for T cells to analyse the different subsets (Naive, CM, EM and EMRA), and to measure senescent and exhausted t cells. I want to...

02 January 2024 5,387 1 View

Which plasmid to use for CRISPR in Lewis Lung Carcinoma and subsequent injection?

Hi, we've been unsuccessful in creating single clones with deletion of a couple of proteins, after testing several sgRNA sequences for each. We are using a lentiviral vector encoding puromycin for...

25 December 2023 2,901 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View