Hi everyone,
For my neurogenesis project, I'd like to inject young pups (P14) with EdU for a long-term chase (P56). So far, my idea was to perform two days of pulse with 2 injections per day (P14 and P15) with a dose of 100 mg/kg per injection. Given how small the pups are at that time, it roughly corresponds to 50 - 60 µL of a solution of 10 mg/mL EdU. However, I'd like to know how to prepare such a solution. I've read protocols where EdU is first dissolved at 200 mg/mL in DMSO, then diluted in 1X PBS to 10 mg/mL. I am however worried that a 5% DMSO solution might be toxic for young pups. Has anyone ever injected young pups with EdU? Thanks for your help and answers!