Most articles I have read mentioned Hamiltonella to be present in the invasive MED and MEAM species. However, we seem to have detected Hamiltonella in an Asia species. anyone with the same experience?
Even it seems that Hamiltonellena is a recent acquisiton on the MED/MEAN1 clade (actually lost in Q2) I will not discard that it can appear in other species. Sometimes endosymbionts are in low titer and are hard to detect by regular PCR and the primers actualy used. Also, not all the indivuals from the same population need to carry the same edosymbionts andyou can find several combinations. I think you should sequence the full 16 from your Hamiltonella and check how divergente it is from the MEAM1/MED to know if it is a case of horizontal transfer. Also determine which Asia species you have.
Hi diego! Its an Asia II 6 we have. You're quite right, we have variations in the diversity even from individuals belonging to the same population. That's really an interesting answer, thank you! We'll take your suggestion and look into the variations if we can find any similarities.
Hi Justine! From the metastudy o Zchori-Fein etal 2014 (Variations in the identity and complexity of endosymbiont combinations in whitefly hosts. Front Microbiol 5:1–8.) it seems that the common combinations is Wolbachia or Wolbacia plus Arsenophonus. It could be very interesting if you sequence several bacterial universal markers from your Hamiltonella (rpoB and C, groEL, atpD,...) and make a good phylogeny compared with the others. Hamiltonella have several 16S rRNA so an analysis on single copy genes will give you more support. Maybe this database can help you: http://symbiogenomesdb.uv.es/. I wish you will find a very interesting study.
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