Hydroxychloroquine Protonation in Blood and Cells - inconsistent information on claimed alkalinization inside targets, emphasis on Coronavirus?

Some researchers have claimed that Hydroxychloroquine (HCQ) can enter cells and make the environment more "alkaline" by soaking up protons. Many publications discuss only 2 nitrogen atoms that can...

08 August 2020 6,262 3 View

Do rats play a part in Covid-19?

The VIZIONS project in Vietnam in 2018 examined bat and rat urine and faeces. Alpha coronavirus was found in bats, beta in rats, with a comment that rats in China also carry coronavirus. So,...

10 May 2020 7,974 5 View

Organofluorine compounds in Kombucha?

Kombucha, a fermented Tea with low pH, contains substantial Fluoride in the form of Hydrogen Fluoride as well as the ion. Kombucha has been associated with many adverse effects, including acute...

12 February 2020 408 1 View

Could areas of high Fluoride ingestion be more susceptible to Coronavirus outbreaks?

The US Government Comparative Toxicogenomics database shows that Fluoride can inhibit Human immunity to viruses and pneumonia. Angiotensin I-Converting Enzyme (ACE), 2'-5'-Oligoadenylate...

03 February 2020 6,459 4 View

Do you have Fluoride analysis of Psammoma bodies isolated from Cancers or could you have them analyzed now from stored specimens?

I am updating my earlier review on the role of Fluoride doped Hydroxyapatite in Cancer and my current focus is on Psammoma Bodies which have been found, and identifed as high risk, in a very wide...

31 January 2019 3,477 1 View

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01 March 2018 8,712 2 View

Does anyone know of a means for testing worldview assumptions (apart from the IAT) and also assessing worldview (pre-theoretical) transformation?

I am interested in testing worldviews - at the assumption/presupposition/pre-theoretical (Weltanschauung) level. (This is not a test of espoused beliefs.) Harvard's IAT seems to do this in a very...

12 February 2017 8,608 3 View

COVID-19: Are there any promising suggestions for interim medications while a vaccine is being developed?

See...

01 January 1970 2,745 100 View

HIV segments identified with India, Thailand and Kenya in Covid-19 receptor

01 January 1970 7,209 4 View

Beryllium deliberately added to drinking water supplies - Toxic effects and Analytical Techniques

Beryllium is exceedingly toxic and known to interfere with Human enzymes. Analysts have developed techniques to measure Beryllium in parts per Quadrillion. Beryllium is present in Fluoride...

01 January 1970 3,709 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View